Publications

Cell Signaling Engineering and Optogenetics

Zhou, X., Fan, L., Li, P., Shen, K., Lin, M.#, Optical Control of Cell Signaling by Single-chain Photoswitchable Kinases. Science 2017, 355: 836 – 842.

Zhou, X., Chung, H., Lam, A., Lin, M.#, Optical control of protein activity by fluorescent protein domains. Science 2012. 338: 810 - 814.

Zhou, X., Zou, X., Lin, M.#, A single-chain photoswitchable Cas9 architecture for inducible gene editing and transcription. ACS Chemical Biology 2018, Special Issue on the Chemical Biology of CRISPR, 13(2): 443–448.

Zhou, X., Pan, M., Lin, M.#, Investigating neuronal function with optically controllable proteins. Frontiers in Molecular Neuroscience 2015, 8: 37.

Zhou, X., Lin, M.#, Photoswitchable fluorescent proteins: ten years of colorful chemistry and exciting applications. Curr. Opin. Chem. Biol. 2013, 17: 682 – 690.

Biosensor Designs

Elledge, S.*, Zhou, X.*, Byrnes, J., Martinko, A., Lui, I., Pance, K., Lim, S., Glasgow, J., Glasgow, A., Turcios, K., Iyer, N., Torres, L., Peluso, M., Henrich, T., Wang, T., Tato, C., Leung, K., Greenhouse, B., Wells, J.# Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection. Nature Biotechnology 2021, 39: 928–935. *co-first authors.

Peluso M., Takahashi S., Hakim J., Kelly J., Torres L., Iyer N., Turcios K., Janson O., Munter S., Thanh C., Nixon C., Hoh R., Tai V., Fehrman E., Hernandez Y., Spinelli M., Gandhi M., Palafox M., Vallari A., Rodgers M., Prostko J., Hackett J., Trinh L., Wrin T., Petroplolous C., Chiu C., Norris P., DiGermanio C., Stone M., Busch M., Elledge S., Zhou X., Wells J., Shu A., Kurtz T., Pak J., Wu W., Burbelo P., Cohen J., Rutishauser R., Martin J., Deeks S., Henrich T., Rodriguez-Barraquer I., Greenhouse B.# SARS-CoV-2 antibody magnitude and detectability are driven by disease severity, timing, and assay. Science Advances 2021, 7(31): eabh3409.

Byrnes, J., Zhou, X., Lui, I., Elledge, S., Glasgow, J., Lim, S., Loudermilk, R., Chiu, C., Wang, T., Wilson, M., Leung, K., Wells, J.# A competitive SARS-CoV-2 serological assay reveals a majority of patient antibodies targeting the spike receptor binding domain compete for ACE2 binding. mSphere 2020, 5(5): e00802-20.

Zamecnik, C., Rajan, J., Yamauchi, K., Mann, S., Loudermilk, R., Sowa, G., Zorn, K., Alvarenga, B., Gaebler, C., Caskey, M., Stone, M., Norris, P., Gu, W., Chiu, C., Ng, D., Byrnes, J., Zhou, X., Wells, J., Robbiani, DF., Nussenzweig, M., DeRisi, J., Wilson, M.# ReScan, a Multiplex Diagnostic Pipeline, Pans Human Sera for SARS-CoV-2 Antigens. Cell Rep. Med. 2020, 1(7):100123.

Functional Antibody Engineering

Zhou, X., Bracken, C., Zhang, K., Zhou, J., Mou, Y., Wang, L., Cheng, Y., Leung, K., Wells, J.# Targeting phosphotyrosine in native proteins with conditional, bi-specific antibody traps. J. Am. Chem. Soc. 2020. 142(41), 17703-17713.

Mou, Y., Zhou, X., Leung, K., Martinko, A., Yu, J., Chen, W., Mayo, S., Wang, L., Wells, J.# Engineering Improved Antiphosphotyrosine Antibodies Based on an Immunoconvergent Binding Motif. J. Am. Chem. Soc. 2018, 140(48):16615–16624.

Bracken, C., Lim, S., Solomon, P., Rettko, N., Nguyen, D., Zha, B., Schaefer, K., Byrnes, J., Zhou, J., Lui, I., Liu, J., Pance, K., QCRG Structural Biology Consortium, Zhou, X., Leung, K., Wells, J.# Bi-paratopic and multivalent human VH domains neutralize SARS-CoV-2 by targeting distinct epitopes within the ACE2 binding interface of Spike. Nature Chemical Biology, 2021, 17: 113-121 (senior author).

Lim, S., Gramespacher, J., Pance, K.*, Solomon, P., Jin, J., Rettko, N., Lui, I., Elledge, S., Liu, J., Bracken, C., Simmons, G., Zhou, X., Leung, K., Wells, J.# Bi-specific VH-Fab IgGs combining neutralizing and non-neutralizing epitopes to Spike receptor binding domain potently block SARS-CoV-2 infection. mAbs 2021, 13(1): 1893426 (senior author).

Glasgow, A., Glasgow, J., Limonta, D., Solomon, P., Lui, I., Zhang, Y., Nix, M., Rettko, N., Zha, B., Yamin, R., Kao, K., Rosenberg, O., Ravetch, J., Wiita, A., Leung, K., Lim, S., Zhou, X., Hobman, T., Kortemme, T., Wells, J.# Engineered ACE2 receptor traps potently neutralize SARS-CoV-2. PNAS 2020, 117 (45): 28046-28055 (senior author).